Afuco™ Anti-RSV F ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-009)
CAT#: AFC-TAB-009
This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to Human RSV. It is a monoclonal antibody produced by recombinant DNA technology. It is used in the prevention of respiratory syncytial virus (RSV) infections. It is recommended for infants that are high-risk because of prematurity or other medical problems such as congenital heart disease. is a humanized monoclonal antibody (IgG) directed against an epitope in the A antigenic site of the F protein of RSV.
Tested Data
SDS-PAGE
Figure 1 Afuco Anti-RSV F ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-009) in SDS-PAGE
SDS-PAGE analysis of AFC-TAB-009 in reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of AFC-TAB-009 was greater than 95% as determined by SDS-PAGE.
SEC-HPLC
Figure 2 Afuco Anti-RSV F ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-009) in HPLC
The purity of AFC-TAB-009 was greater than 94% as determined by SEC-HPLC
Lectin WB
Figure 3 Afuco Anti-RSV F ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-009) in Lectin WB
Western blot analysis of AFC-TAB-009 was performed and fucose was detected by AOL-Biotin and SA-HRP. The result was negative, which indicates that there is almost no fucose produced.
Lane 1: Reducing AFC-TAB-009 (2 μg)
Lane 2: Reducing positive control antibody (2 μg)
Lectin ELISA
Figure 4 Afuco Anti-RSV F ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-009) in Lectin ELISA
Lectin ELISA analysis of AFC-TAB-009 was conducted and fucose was detected using AOL-Biotin and SA-HRP
The result indicates that there is almost no fucose produced.
WB
Figure 5 Afuco Anti-RSV F ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-009) in WB
Western blot analysis of AFC-TAB-009 was performed by loading RSV F.
AFC-TAB-009 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-anti-human IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
ELISA
Figure 6 Afuco Anti-RSV F ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-009) in ELISA
ELISA analysis of AFC-TAB-009 was performed by coating with RSV F.
The secondary antibody: HRP-anti-human IgG
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Humanized (from mouse)
- Type
- ADCC enhanced antibody
- Specificity
- Tested positive against native RSV antigen
- Species Reactivity
- RSV
- Applications
- IF, IP, Neut, FuncS, ELISA, FC
- Conjugate
- Unconjugated
- Trade name
- Synagis
- Generic Name
- Palivizumab
- Related Disease
- Respiratory Syncytial Virus (RSV) disease
Product Property
- Purity
- >95% by SDS-PAGE
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- RSV; F; Fusion protein (F); NP_044596.1; UniProt ID: O09720; O09720; Entrez: 1494475; Entrez Gene: 1494475; Gene ID: 1494475;
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# AFC-TAB-009, RRID: AB_3111281)
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Biosimilar Overview
Please refer to Palivizumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Palivizumab.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-009 | Human Anti-RSV Recombinant Antibody (TAB-009) | IF, IP, Neut, FuncS, ELISA, FC, WB | IgG1 - kappa |
| TAB-709 | Anti-RSV glycoprotein F Recombinant Antibody (TAB-709) | IF, IP, Neut, FuncS, ELISA, FC, WB | IgG1 - kappa |
| MRO-1209LC | Anti-HRSV F Recombinant Antibody (MEDI-493) | ELISA, Neut, PK | Humanized antibody |
| MRO-1209LC-S(P) | Anti-HRSV F Recombinant Antibody scFv Fragment (MEDI-493) | ELISA | Humanized antibody |
| MRO-1209LC-F(E) | Anti-HRSV F Recombinant Antibody Fab Fragment (MEDI-493) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-358CT | Human Anti-RSV F Recombinant Antibody (TAB-358CT) | ELISA, WB, Neut, IF, FC | Human IgG1, κ |
| TAB-361CT | Human Anti-RSV F Recombinant Antibody (TAB-361CT) | ELISA, WB, Neut, IP | Human antibody |
| TAB-362CT | Human Anti-RSV F Recombinant Antibody (TAB-362CT) | ELISA, WB, Neut, IP | Human antibody |
| TAB-363CT | Human Anti-RSV F Recombinant Antibody (TAB-363CT) | ELISA, WB, Neut, IP | Human antibody |
| TAB-364CT | Human Anti-RSV F Recombinant Antibody (TAB-364CT) | ELISA, WB, Neut, IP | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-878LC | Human Anti-RSV F Recombinant Antibody (MRO-878LC) | ELISA, Neut | Human IgG |
| PABC-276 | Recombinant Mouse Anti-RSV F Antibody (PABC-276) | ELISA, Neut, DB, FC | Mouse IgG |
| PABC-494 | Recombinant Human Anti-RSV F Antibody (14N4) | SPR, ELISA, Neut | Human IgG1, κ |
| HPAB-0105-WJ | Human Anti-RSV F Recombinant Antibody (HPAB-0105-WJ) | ELISA, WB, Neut, IF, FC, Inhib, PK, IHC, DB | Human IgG |
| PABS-0157 | Human Anti-RSV F Recombinant Antibody (clone C57) | FC, ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-878LC-S(P) | Human Anti-RSV F Recombinant Antibody; scFv Fragment (MRO-878LC-S(P)) | ELISA, Neut | Human scFv |
| HPAB-0293CQ-S(P) | Human Anti-RSV F Recombinant Antibody; scFv Fragment (HPAB-0293CQ-S(P)) | ELISA, Neut | Human scFv |
| PSBC-494 | Recombinant Human Anti-RSV F Antibody scFv Fragment (14N4) | ELISA, Neut | Human scFv |
| HPAB-0105-WJ-S(P) | Human Anti-RSV F Recombinant Antibody; scFv Fragment (HPAB-0105-WJ-S(P)) | ELISA, WB | Human scFv |
| PABJ-0166-S(P) | Human Anti-RSV F Recombinant Antibody scFv Fragment (PABJ-0166-S(P)) | ELISA, Neut | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-878LC-F(E) | Human Anti-RSV F Recombinant Antibody; Fab Fragment (MRO-878LC-F(E)) | ELISA, Neut | Human Fab |
| HPAB-0293CQ-F(E) | Human Anti-RSV F Recombinant Antibody; Fab Fragment (HPAB-0293CQ-F(E)) | ELISA, Neut | Human Fab |
| PFBC-276 | Recombinant Mouse Anti-RSV F Antibody Fab Fragment (PFBC-276) | ELISA, Neut | Mouse Fab |
| PFBC-494 | Recombinant Human Anti-RSV F Antibody Fab Fragment (14N4) | ELISA, Neut | Human Fab |
| PABJ-0166-F(E) | Human Anti-RSV F Recombinant Antibody Fab Fragment (PABJ-0166-F(E)) | ELISA, Neut | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1210LC | Anti-HRSV F Recombinant Antibody (C11) | ELISA | Mouse Antibody |
| MRO-1211LC | Anti-HRSV F Recombinant Antibody (C13) | ELISA | Mouse Antibody |
| MRO-1210LC-S(P) | Anti-HRSV F Recombinant Antibody scFv Fragment (C11) | ELISA | Mouse Antibody |
| MRO-1211LC-S(P) | Anti-HRSV F Recombinant Antibody scFv Fragment (C13) | ELISA | Mouse Antibody |
| MRO-1210LC-F(E) | Anti-HRSV F Recombinant Antibody Fab Fragment (C11) | ELISA | Mouse Antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-709 | Afuco™ Anti-RSV F ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-709) | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YC250 | H-2Kd/Respiratory syncytial virus Fusion protein (F) (KYKNAVTEL) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY106 | Mouse Anti-RSV F (clone 101F) scFv-Fc Chimera | ELISA, Neut | Mouse IgG1, scFv-Fc |
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